Protein Molecular Weight Markers
Cayman Chemical VItelogenIncod PolycLNal 500uL
Isoprostanesprostaglandin PG-like productsof freeradicalinducedlipidperoxidation.{320 Although the isoprostanes derived from arachidonic acid are the best characterized many other polyunsaturated fatty acids can form isoprostanes.{7323} (±)5-iPF2a-VI is one of dozens of possible stereo- and regioisomeric isoprostanes which can be formed from arachidonic acid. To date the most extensively studied of these is 8-isoprostane (8-epi-PGF2a iPF2a-III).{30645011} However 8-isoprostane is a minor isoprostane constituent when compared to some of the other isomers which form in natural conditions of oxidative stress.{8367} (±)5-iPF2a-VI is an isoprostane from the unique Type VI class of isoprostanes. This class has been shown to be one of the major isoprostane products in contrast to 8-isoprostane. In addition to being produced in greater abundance than 8-isoprostane Type VI isoprostanes form internal lactones which facilitates their extraction purification fro biological samples8367940869106390
Proteintech Group Inc Prestained protein marker
The PL00001 Regular Range Protein Marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins.
Sigma Aldrich Fine Chemicals Biosciences Western BlockerTM Solution
Western Blocker SOLUTIONon is used to block both PVDf and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Sigma Aldrich Fine Chemicals Biosciences ECLTM Start Western Blotti
Amersham ECL start is a chemiluminescent detection reagent in the Amersham ECL product family that is recommended for confirmatory analysis and detection of high to medium levels of endogenous proteins. Amersham ECL start has a long signal duration which enables multiple exposures of the membrane plus a convenient time window between experiment and analysis.
Sigma Aldrich Fine Chemicals Biosciences VivaspinR 500 30 kDa MWCO
Designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.Vivaspin(R) sample concentrators are designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane leaving a more concentrated sample in the upper chamber.Vivaspin(R) sample concentrators cater for sample volumes from 100 mul to 20 ml with a range of molecular weight cutoff values from Mr 3 000 to 100 000. for maximum recovery select a MWCO at
Sigma Aldrich Fine Chemicals Biosciences VivaspinR 500 100 kDa MWCO
Designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.Vivaspin(R) sample concentrators are designed for fast nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane leaving a more concentrated sample in the upper chamber.Vivaspin(R) sample concentrators cater for sample volumes from 100 mul to 20 ml with a range of molecular weight cutoff values from Mr 3 000 to 100 000. for maximum recovery select a MWCO at
Sigma Aldrich Fine Chemicals Biosciences fluorescent IEf-Marker pI
filtered through a 0.45 mum membrane filter
Biolegend Purified anti-mouse Podoplanin
Purified anti-mouse Podoplanin Recombinant [QA20A17]; Isotype: Rat IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg
Gold Biotechnology Inc BLUEstain™ Protein ladder, 11-245 kDa 500 µL
The BLUEstainTM Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from ~11 to 245 kDa. Proteins are covalently coupled with a bluechromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstainTM Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.